pires 2 egfp vector Search Results


pires-egfp vector  (Becton Dickinson)
90
Becton Dickinson pires-egfp vector
Pires Egfp Vector, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pires+2+egfp+vector/pmc04487042-52-8-10?v=Becton+Dickinson
Average 90 stars, based on 1 article reviews
pires-egfp vector - by Bioz Stars, 2026-08
90/100 stars
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pires2-egfp-tim-3-ig vector  (PolyScience)
90
PolyScience pires2-egfp-tim-3-ig vector
Comparison of <t>Tim-3</t> aminoacid sequences of BALB/c with three different mouse strains. cDNA of Tim-3 was cloned from activated splenocytes of BALB/c mouse, nucleotide sequences were analyzed and deduced amino acid sequences were shown. Accession numbers of BALB/c Tim-3 are AY553334.1 for nucleotide sequences and AAS5931.1 for amino acid sequences. Accession numbers of C57BL/6, DBA/2 and AKR Tim-3 are BAE28574.1, AAL35776.1 and AAL65156.1, respectively.
Pires2 Egfp Tim 3 Ig Vector, supplied by PolyScience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pires+2+egfp+vector/pmc02803304-44-16-21?v=PolyScience
Average 90 stars, based on 1 article reviews
pires2-egfp-tim-3-ig vector - by Bioz Stars, 2026-08
90/100 stars
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Comparison of Tim-3 aminoacid sequences of BALB/c with three different mouse strains. cDNA of Tim-3 was cloned from activated splenocytes of BALB/c mouse, nucleotide sequences were analyzed and deduced amino acid sequences were shown. Accession numbers of BALB/c Tim-3 are AY553334.1 for nucleotide sequences and AAS5931.1 for amino acid sequences. Accession numbers of C57BL/6, DBA/2 and AKR Tim-3 are BAE28574.1, AAL35776.1 and AAL65156.1, respectively.

Journal: Immune Network : Official Journal of the Korean Society for Immunology and Biological Response Modifiers

Article Title: The Binding Properties of Glycosylated and Non-Glycosylated Tim-3 Molecules on CD4 + CD25 + T Cells

doi: 10.4110/in.2009.9.2.58

Figure Lengend Snippet: Comparison of Tim-3 aminoacid sequences of BALB/c with three different mouse strains. cDNA of Tim-3 was cloned from activated splenocytes of BALB/c mouse, nucleotide sequences were analyzed and deduced amino acid sequences were shown. Accession numbers of BALB/c Tim-3 are AY553334.1 for nucleotide sequences and AAS5931.1 for amino acid sequences. Accession numbers of C57BL/6, DBA/2 and AKR Tim-3 are BAE28574.1, AAL35776.1 and AAL65156.1, respectively.

Article Snippet: In order to produce CD4 + Tim-3-Ig protein, Chinese hamster ovary (CHO) cells were transfected with pIRES2-EGFP-Tim-3-Ig vector using Polyethylenimine (PEI, Polyscience, PA USA).

Techniques: Clone Assay

Analysis of Tim-3-Ig purified from CHO cell and E. coli culture. (A) Tim-3-Ig was purified from culture supernatant of CHO cells transfected with pIRES2-EGFP-Tim-3-Ig using protein A affinity column chromatography and subjected to SDS-PAGE and Western blot using peroxidase conjugated anti-human IgG antibody and ECL detection system. (B) Tim-3-Ig was purified from culture supernatant of E. coli transformed with pIg20-Tim-3-Ig and subjected to SDS-PAGE and Western blot using peroxidase conjugated anti-human IgG antibody and ECL detection system.

Journal: Immune Network : Official Journal of the Korean Society for Immunology and Biological Response Modifiers

Article Title: The Binding Properties of Glycosylated and Non-Glycosylated Tim-3 Molecules on CD4 + CD25 + T Cells

doi: 10.4110/in.2009.9.2.58

Figure Lengend Snippet: Analysis of Tim-3-Ig purified from CHO cell and E. coli culture. (A) Tim-3-Ig was purified from culture supernatant of CHO cells transfected with pIRES2-EGFP-Tim-3-Ig using protein A affinity column chromatography and subjected to SDS-PAGE and Western blot using peroxidase conjugated anti-human IgG antibody and ECL detection system. (B) Tim-3-Ig was purified from culture supernatant of E. coli transformed with pIg20-Tim-3-Ig and subjected to SDS-PAGE and Western blot using peroxidase conjugated anti-human IgG antibody and ECL detection system.

Article Snippet: In order to produce CD4 + Tim-3-Ig protein, Chinese hamster ovary (CHO) cells were transfected with pIRES2-EGFP-Tim-3-Ig vector using Polyethylenimine (PEI, Polyscience, PA USA).

Techniques: Purification, Transfection, Affinity Column, Chromatography, SDS Page, Western Blot, Transformation Assay

The binding activity of Tim-3-Ig to CD4 + CD25 + T cells. Mouse CD4 + T cells were labeled with FITC conjugated anti-CD4 Ab, PE-conjugated anti-CD25 Ab and Tim-3-Ig purified either from CHO cells (A) or from E. coli (B). Subsequently, cells were stained with biotin-conjugated anti-human IgG Ab and Streptavidin-PerCP. Cells were gated on CD4 CD25 expression and the binding activity of Tim-3-Ig to these cells was analyzed by flow cytometry.

Journal: Immune Network : Official Journal of the Korean Society for Immunology and Biological Response Modifiers

Article Title: The Binding Properties of Glycosylated and Non-Glycosylated Tim-3 Molecules on CD4 + CD25 + T Cells

doi: 10.4110/in.2009.9.2.58

Figure Lengend Snippet: The binding activity of Tim-3-Ig to CD4 + CD25 + T cells. Mouse CD4 + T cells were labeled with FITC conjugated anti-CD4 Ab, PE-conjugated anti-CD25 Ab and Tim-3-Ig purified either from CHO cells (A) or from E. coli (B). Subsequently, cells were stained with biotin-conjugated anti-human IgG Ab and Streptavidin-PerCP. Cells were gated on CD4 CD25 expression and the binding activity of Tim-3-Ig to these cells was analyzed by flow cytometry.

Article Snippet: In order to produce CD4 + Tim-3-Ig protein, Chinese hamster ovary (CHO) cells were transfected with pIRES2-EGFP-Tim-3-Ig vector using Polyethylenimine (PEI, Polyscience, PA USA).

Techniques: Binding Assay, Activity Assay, Labeling, Purification, Staining, Expressing, Flow Cytometry

Production of Tim-3-Ig N-glycosylation mutants. (A) Schematic representation and sequence analysis of Tim-3-Ig N-glycosylation mutants, N53Q and N100Q. (B) Tim-3-Ig N-glycosylation mutants were purified from CHO cell culture using affinity column chromatography and subjected to SDS-PAGE analysis and Western blot using peroxidase conjugated anti-human Ig antibody and ECL detection system.

Journal: Immune Network : Official Journal of the Korean Society for Immunology and Biological Response Modifiers

Article Title: The Binding Properties of Glycosylated and Non-Glycosylated Tim-3 Molecules on CD4 + CD25 + T Cells

doi: 10.4110/in.2009.9.2.58

Figure Lengend Snippet: Production of Tim-3-Ig N-glycosylation mutants. (A) Schematic representation and sequence analysis of Tim-3-Ig N-glycosylation mutants, N53Q and N100Q. (B) Tim-3-Ig N-glycosylation mutants were purified from CHO cell culture using affinity column chromatography and subjected to SDS-PAGE analysis and Western blot using peroxidase conjugated anti-human Ig antibody and ECL detection system.

Article Snippet: In order to produce CD4 + Tim-3-Ig protein, Chinese hamster ovary (CHO) cells were transfected with pIRES2-EGFP-Tim-3-Ig vector using Polyethylenimine (PEI, Polyscience, PA USA).

Techniques: Sequencing, Purification, Cell Culture, Affinity Column, Chromatography, SDS Page, Western Blot

The binding activity of Tim-3-Ig N-glycosylation mutants to CD4 + CD25 + T cells. Mouse CD4 + T cells were labeled with FITC conjugated anti-CD4 Ab, PE-conjugated anti-CD25 Ab and either Tim-3-Ig wild type or Tim-3-Ig N-glycosylation mutants. Subsequently, these cells were incubated with biotin-conjugated anti-human IgG Ab and Streptavidin-PerCP. Cells were gated on CD4 CD25 expression and the binding activity of Tim-3-Ig to these cells was analyzed by flow cytometry.

Journal: Immune Network : Official Journal of the Korean Society for Immunology and Biological Response Modifiers

Article Title: The Binding Properties of Glycosylated and Non-Glycosylated Tim-3 Molecules on CD4 + CD25 + T Cells

doi: 10.4110/in.2009.9.2.58

Figure Lengend Snippet: The binding activity of Tim-3-Ig N-glycosylation mutants to CD4 + CD25 + T cells. Mouse CD4 + T cells were labeled with FITC conjugated anti-CD4 Ab, PE-conjugated anti-CD25 Ab and either Tim-3-Ig wild type or Tim-3-Ig N-glycosylation mutants. Subsequently, these cells were incubated with biotin-conjugated anti-human IgG Ab and Streptavidin-PerCP. Cells were gated on CD4 CD25 expression and the binding activity of Tim-3-Ig to these cells was analyzed by flow cytometry.

Article Snippet: In order to produce CD4 + Tim-3-Ig protein, Chinese hamster ovary (CHO) cells were transfected with pIRES2-EGFP-Tim-3-Ig vector using Polyethylenimine (PEI, Polyscience, PA USA).

Techniques: Binding Assay, Activity Assay, Labeling, Incubation, Expressing, Flow Cytometry